Research

Cellular oxidants such as hydrogen peroxide and superoxide are generated by ligand binding of numerous types of surface receptors, including cytokine and growth factor receptors. Redox couples provide a means of translating the presence of ROS into useful signals in the cell. For example, thioredoxin and glutathione-regulated post-translational modifications of proteins (disulfide bonds and S-glutathionylation, respectively) have been shown to functionally alter the activity of some proteins. While some proteins have been investigated in depth to understand this relationship, how redox-related effects systemically influence the regulation of receptor signaling pathways is unknown. There are challenges in quantifying reversible protein oxidation events and discerning the effects of one redox couple from another. These challenges have compounded the difficulties in understanding the role of cellular oxidation in signaling, mandating a modeling-based approach for gaining insight into these biological processes.

Our lab uses computational modeling and wet-lab experimentation to investigate how oxidative thiol modification of proteins influences the information flow from receptors to the nucleus. We study these effects primarily via interleukin, TCR, or TNF-alpha signaling, physiological cues that induce cellular oxidation. Please read more about our various projects below.

3/3/17

Undergraduate Yeshy Manoharan is named the inaugural Stephen E. Brossette Scholar for his interdisciplinary research in biology and computing. Congratulations, Yeshy!

2/18/17

Melissa will be presenting our EBICS work in a special session on cellular machines at AAAS in Boston.

1/25/17

Two of our undergraduate BME researchers, Melody Shao and Nicholas Peterman, received Presidential Undergraduate Research Awards for their projects this spring. Congratulations to both of them for this honor!

9/28/16
Dr. Chenyi Pan wins one of the poster awards at the Georgia Bio Innovation Summit!

4/18/16
Ariel Kniss-James defends her Ph.D. with the dissertation titled "Analysis of Calcium and Hydrogen Peroxide Frequency Responses in T cells at Single-Cell Resolution via Microfluidic Traps". Congratulations on an impressive body of work!

Kniss-James, A.S., Rivet, C.A., Chingozha, L., Lu, H., Kemp, M.L. “Single-cell resolution of intracellular T cell Ca2+ dynamics in response to frequency-based stimulation”. Integrative Biology, DOI: 10.1039/C6IB00186F, 2017.

 

Prasanphanich, A.F., White, D.E., Gran, M.E., Kemp, M.L. “Kinetic Modeling of ABCG2 Transporter Heterogeneity: A Quantitative, Single-Cell Analysis of the Side Population Assay”. PLoS Computational Biology, Nov 16;12(11):e1005188, 2016.

 

*Rivet, C.A., *Kniss, A., Gran, M.A., Potnis, A., Hill, A., Lu, H., Kemp, M.L. “Calcium dynamics of ex vivo long-term cultured CD8+ T cells are regulated by age related changes in redox metabolism”. PLoS One,11(8):e0159248, 2016.