Research

Cellular oxidants such as hydrogen peroxide and superoxide are generated by ligand binding of numerous types of surface receptors, including cytokine and growth factor receptors. Redox couples provide a means of translating the presence of ROS into useful signals in the cell. For example, thioredoxin and glutathione-regulated post-translational modifications of proteins (disulfide bonds and S-glutathionylation, respectively) have been shown to functionally alter the activity of some proteins. While some proteins have been investigated in depth to understand this relationship, how redox-related effects systemically influence the regulation of receptor signaling pathways is unknown. There are challenges in quantifying reversible protein oxidation events and discerning the effects of one redox couple from another. These challenges have compounded the difficulties in understanding the role of cellular oxidation in signaling, mandating a modeling-based approach for gaining insight into these biological processes.

Our lab uses computational modeling and wet-lab experimentation to investigate how oxidative thiol modification of proteins influences the information flow from receptors to the nucleus. We study these effects primarily via interleukin, TCR, or TNF-alpha signaling, physiological cues that induce cellular oxidation. Please read more about our various projects below.

7/28/17

Sarah, Chad, Jakari, and Melissa attend the annual EBICS retreat at Calloway Gardens

7/1/17

Our lab is awarded an IBB seed grant in collaboration with Facundo Fernandez in Chemistry. Dr. Li Li and Sarah Seals will have internal funding to continue their amazing collaboration using the latest Bruker Rapiflex MALDI imager on campus! Here is a press release!

6/13/17

Kendrecus Weldon has joined the Kemp lab as our newest Project ENGAGES student and is mentored by Sarah Seals. Read here for more information on this amazing program. Our lab is proud to be participants!

Lewis J.E., Costantini F., Mims J., Chen X., Furdui C., Boothman D.A., Kemp M.L. “Genome-scale modeling of NADPH-driven β-lapachone sensitization in head and neck squamous cell carcinoma”. Antioxidant Redox Signaling. 2017 (in press).

Kniss-James, A.S., Rivet, C.A., Chingozha, L., Lu, H., Kemp, M.L. “Single-cell resolution of intracellular T cell Ca2+ dynamics in response to frequency-based stimulation”. Integrative Biology, DOI: 10.1039/C6IB00186F, 2017.

Prasanphanich, A.F., White, D.E., Gran, M.E., Kemp, M.L. “Kinetic Modeling of ABCG2 Transporter Heterogeneity: A Quantitative, Single-Cell Analysis of the Side Population Assay”. PLoS Computational Biology, Nov 16;12(11):e1005188, 2016.