Aging, stem cell differentiation, cancer metastasis, and inflammation rely on progressive changes in metabolism. It is not fully clear how and why metabolic changes take place, but the consequence is an increase in the levels of reactive oxygen species. Collectively, the accumulation of these molecules is known as cellular oxidation, and pathological levels are referred to as oxidative stress. Our lab develops systems biology tools for analyzing how cellular oxidation influences cellular decisions. We are interested in the collective behavior that arises during stem cell differentiation, immune cell responses, or drug treatments from metabolic diversity in individual cells. Because of the numerous biochemical reactions involved, we use computational modeling to investigate how protein networks are regulated in the presence of reactive oxygen species by changes in activity and/or function of redox-sensitive proteins. Experimentally, we are developing novel high-throughput single cell techniques for the detection and quantification of intracellular oxidation.

We are located in the Wallace H. Coulter Department of Biomedical Engineering administered jointly between Georgia Tech and Emory University School of Medicine. The lab is located on the Georgia Tech campus in the Engineered Biosystems Building.

Dr. Chenyi Pan wins one of the poster awards at the Georgia Bio Innovation Summit!

A multi-year team effort by Catherine, Ariel, Maggie, Abby, and Anish has finally come to fruition. Our publication "Calcium dynamics of ex vivo long-term cultured CD8+ T cells are regulated by changes in redox metabolism" is now available on PLoS One's website.

Ariel Kniss-James defends her Ph.D. with the dissertation titled "Analysis of Calcium and Hydrogen Peroxide Frequency Responses in T cells at Single-Cell Resolution via Microfluidic Traps". Congratulations on an impressive body of work!

Integrative Biology selects our paper on multicellular patterning as one of their top cancer-related articles. Read more about it here!

*Rivet, C.A., *Kniss, A., Gran, M.A., Potnis, A., Hill, A., Lu, H., Kemp, M.L. “Calcium dynamics of ex vivo long-term cultured CD8+ T cells are regulated by age related changes in redox metabolism”. PLoS One,11(8):e0159248, 2016.

*Warren, E.A.K., *Netterfield, et al. “Spatially-resolved Intracellular Sensing of Hydrogen Peroxide in Living Cells”. Scientific Reports, 5:16929, DOI: 10.1038/srep16929, 2015.

Dwivedi, G., et al. “Dynamic Redox Regulation of IL-4 Signaling”. PLoS Computational Biology, DOI:10.1371/journal.pcbi.1004582, 2015.

White, D.E., et al. “Quantitative Multivariate Analysis of Dynamic Multicellular Morphogenic Trajectories”, Integrative Biology, DOI:10.1039/c5ib00072f, 2015.