Aging, stem cell differentiation, cancer metastasis, and inflammation rely on progressive changes in metabolism. It is not fully clear how and why metabolic changes take place, but the consequence is an increase in the levels of reactive oxygen species. Collectively, the accumulation of these molecules is known as cellular oxidation, and pathological levels are referred to as oxidative stress. Our lab develops systems biology tools for analyzing how cellular oxidation influences cellular decisions. We are interested in the collective behavior that arises during stem cell differentiation, immune cell responses, or drug treatments from metabolic diversity in individual cells. Because of the numerous biochemical reactions involved, we use computational modeling to investigate how protein networks are regulated in the presence of reactive oxygen species by changes in activity and/or function of redox-sensitive proteins. Experimentally, we are developing novel high-throughput single cell techniques for the detection and quantification of intracellular oxidation.

We are located in the Wallace H. Coulter Department of Biomedical Engineering administered jointly between Georgia Tech and Emory University School of Medicine. The lab is located on the Georgia Tech campus in the Engineered Biosystems Building.

Ariel Kniss-James defends her Ph.D. with the dissertation titled "Analysis of Calcium and Hydrogen Peroxide Frequency Responses in T cells at Single-Cell Resolution via Microfluidic Traps". Congratulations on an impressive body of work!


Integrative Biology selects our paper on multicellular patterning as one of their top cancer-related articles. Read more about it here!


Georgia Tech News Office issues a press release on our Scientific Reports paper. You can read it here


Our paper describing a microtubule-bound hydrogen peroxide sensor in collaboration with the Payne lab is accepted to Scientific Reports. Co-first author is undergraduate Tatiana Netterfeld, the first time an undergrad in the lab has headed a manuscript. Congratulations to Tatiana and Saheli Sarkar for this outstanding work!


October has been a big month for the lab. Two major NIH awards with Kemp lab participation are announced. A transformative R01 led by Tom Barker in pulmonary fibrosis (press release here) and the National Exposure Assessment Laboratory at Emory (NEALE), part of the new NIEHS Children's Health Exposure Analysis Resource (press release here). We are excited to be participants in the Director's Common Fund for High Risk High Reward research once more!

*Warren, E.A.K., *Netterfield, et al. “Spatially-resolved Intracellular Sensing of Hydrogen Peroxide in Living Cells”. Scientific Reports, 5:16929, DOI: 10.1038/srep16929, 2015.

Dwivedi, G., et al. “Dynamic Redox Regulation of IL-4 Signaling”. PLoS Computational Biology, DOI:10.1371/journal.pcbi.1004582, 2015.

White, D.E., et al. “Quantitative Multivariate Analysis of Dynamic Multicellular Morphogenic Trajectories”, Integrative Biology, DOI:10.1039/c5ib00072f, 2015.

*He L, *Kniss A, et al. "An automated platform enabling dynamic stimuli delivery and cellular response readout for high-throughput single-cell signaling studies". Lab on a Chip, 15(6):1497-507, 2015.